Amphiphysin 2 (Bin1) and T-tubule biogenesis in muscle.
Science
; 297(5584): 1193-6, 2002 Aug 16.
Article
in En
| MEDLINE
| ID: mdl-12183633
ABSTRACT
In striated muscle, the plasma membrane forms tubular invaginations (transverse tubules or T-tubules) that function in depolarization-contraction coupling. Caveolin-3 and amphiphysin were implicated in their biogenesis. Amphiphysin isoforms have a putative role in membrane deformation at endocytic sites. An isoform of amphiphysin 2 concentrated at T-tubules induced tubular plasma membrane invaginations when expressed in nonmuscle cells. This property required exon 10, a phosphoinositide-binding module. In developing myotubes, amphiphysin 2 and caveolin-3 segregated in tubular and vesicular portions of the T-tubule system, respectively. These findings support a role of the bilayer-deforming properties of amphiphysin at T-tubules and, more generally, a physiological role of amphiphysin in membrane deformation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Muscle, Skeletal
/
Cell Membrane Structures
/
Muscle Development
/
Nerve Tissue Proteins
Limits:
Animals
Language:
En
Journal:
Science
Year:
2002
Type:
Article
Affiliation country:
United States