Molecular characterization of regulatory elements controlling expression of the Bacillus subtilis recA+ gene.
Biochimie
; 74(7-8): 755-62, 1992.
Article
in En
| MEDLINE
| ID: mdl-1391055
ABSTRACT
Expression of the Bacillus subtilis recA gene is induced following DNA damage as well as during the development of the competent state. DNA damage-induction of the recA gene occurs by a RecA-dependent mechanism, whereas competence-induction occurs by a RecA-independent mechanism. To examine the molecular mechanisms that control the expression of the recA gene, a deletion analysis of the recA promoter region was performed. A regulatory region that is required for repression of recA expression was identified upstream of the recA promoter. Deletion of this regulatory region derepressed expression and abolished damage-induction of the recA promoter. Within this region are sequences similar to the consensus sequence that has been identified within DNA damage-inducible promoter regions of other B subtilis genes. Another regulatory region was identified that is required for the RecA-independent, competence-specific induction of the recA gene. Deletion of these sequences significantly reduced competence-induction of the recA promoter.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rec A Recombinases
/
Bacillus subtilis
/
Gene Expression Regulation, Bacterial
/
Regulatory Sequences, Nucleic Acid
Language:
En
Journal:
Biochimie
Year:
1992
Type:
Article