A system based on specific protein-RNA interactions for analysis of target protein-protein interactions in vitro: successful selection of membrane-bound Bak-Bcl-xL proteins in vitro.

Sawata, Shinya Y; Suyama, Eigo; Taira, Kazunari

Sawata, Shinya Y; Suyama, Eigo; Taira, Kazunari.

Affiliation

Sawata SY; Gene Function Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba Science City 305-8562, Japan.

Protein Eng Des Sel ; 17(6): 501-8, 2004 Jun.

Article in En | MEDLINE | ID: mdl-15292519

Subject(s)

Membrane Proteins/metabolism; Protein Interaction Mapping/methods; Proto-Oncogene Proteins c-bcl-2/metabolism; RNA-Binding Proteins/metabolism; Ribosomes/metabolism; Capsid Proteins/genetics; Cell-Free System/metabolism; DNA/genetics; Glutathione/chemistry; Humans; Membrane Proteins/genetics; Microscopy, Atomic Force; Plasmids/genetics; Polymerase Chain Reaction; Polyribosomes/ultrastructure; Protein Biosynthesis; Proto-Oncogene Proteins c-bcl-2/genetics; RNA, Messenger/genetics; RNA-Binding Proteins/genetics; Reverse Transcriptase Polymerase Chain Reaction; Sepharose/chemistry; Tetrahydrofolate Dehydrogenase/genetics; Transcription, Genetic; bcl-2 Homologous Antagonist-Killer Protein; bcl-X Protein

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Collection: 01-internacional Database: MEDLINE Main subject: Ribosomes / RNA-Binding Proteins / Proto-Oncogene Proteins c-bcl-2 / Protein Interaction Mapping / Membrane Proteins Limits: Humans Language: En Journal: Protein Eng Des Sel Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2004 Type: Article Affiliation country: Japan

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