Adipose development: from stem cell to adipocyte.
Crit Rev Biochem Mol Biol
; 40(4): 229-42, 2005.
Article
in En
| MEDLINE
| ID: mdl-16126487
ABSTRACT
Cell culture models have been developed to study commitment and subsequent differentiation of preadipocytes into adipocytes. Bone morphogenetic protein 4 commits mesenchymal stem cells to the adipose lineage. Other factors, including Wnt signaling, cell density, and cell shape, play a role in lineage commitment. Following commitment to the adipose lineage, growth-arrested preadipocytes can differentiate to adipocytes by treatment with insulin-like growth factor 1, glucocorticoid and an agent that increases cAMP level. This process is characterized by a rapid and transient increase in CCAAT/enhancer binding protein (C/EBP) beta and synchronous re-entry into the cell cycle. Acquisition of DNA-binding by C/EBPbeta occurs after the transcription factor becomes phosphorylated. The cells enter a growth-arrested state and begin terminal differentiation. C/EBPalpha, peroxisome proliferator-activated receptor gamma, and adipocyte determination, and differentiation-dependent factor 1 coordinate the expression of genes that create and maintain the adipocyte phenotype.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stem Cells
/
Adipose Tissue
/
Adipocytes
/
Cell Lineage
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Crit Rev Biochem Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Year:
2005
Type:
Article
Affiliation country:
United States