CRTH2-dependent, STAT6-independent induction of cedar pollen dermatitis.
Clin Exp Allergy
; 38(8): 1357-66, 2008 Aug.
Article
in En
| MEDLINE
| ID: mdl-18477017
ABSTRACT
BACKGROUND:
Airborne contact dermatitis to cedar pollen is a recently identified disease that generally affects individuals with cedar pollinosis of the nasal and/or ocular symptoms, as well as some patients with atopic dermatitis.OBJECTIVE:
To elucidate the pathological mechanisms of cedar pollen dermatitis.METHODS:
We established a mouse model of cedar pollen dermatitis by epicutaneous sensitization with Japanese cedar pollen antigen (Ag).RESULTS:
Histologically, there was marked dermal cellular infiltrate, including eosinophils and mast cells, with epidermal thickening. The induction of dermatitis was accompanied by production of cedar pollen-specific IgE. In the lesional skin, IL-13, IL-18, eotaxin/chemokine (C-C motif) ligand (CCL) 11, regulated upon activation, normal T cell expressed and secreted/CCL5, macrophage-derived chemokine/CCL22 and thymus and activation-regulated chemokine/CCL17, but not IL-4 and IFN-gamma, were produced. Mast cell-deficient WBB6F1-W/W(v) mice failed to develop cedar pollen dermatitis, although regional lymph node cells proliferated in response to Cryptomeria japonica (Cry j) 1 and Cry j2 Ags in vitro. Surprisingly, the induction of dermatitis was independent of STAT6/IgE. In contrast, mice deficient in CRTH2, a receptor for prostaglandin D2 (PGD2), showed diminished inflammation. Consistent with this, ramatroban, a CRTH2 antagonist, significantly inhibited inflammatory cell infiltration.CONCLUSION:
These data suggest that PGD2-CRTH2 signalling contributes to inflammation in cedar pollen dermatitis, and unlike cedar pollinosis of the nasal mucosa, STAT6 is not a therapeutic target for treatment.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pollen
/
Receptors, Prostaglandin
/
Receptors, Immunologic
/
Dermatitis, Allergic Contact
/
Cryptomeria
/
STAT6 Transcription Factor
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Clin Exp Allergy
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
2008
Type:
Article
Affiliation country:
Japan