Influence of the poly(A) tail and putative polyadenylation signal on the infectivity of white clover mosaic potexvirus.
Virology
; 182(1): 61-7, 1991 May.
Article
in En
| MEDLINE
| ID: mdl-2024479
ABSTRACT
A series of mutations has been constructed in a cDNA clone of white clover mosaic virus (WCIMV) which decreases the poly(A) tail length of run-off transcripts from 74 to 27, 10 or zero 3'-terminal (A) residues. Although transcripts with short poly(A) tails were less infectious than wild-type RNA, complete removal of the (A) tail did not abolish infectivity. Addition of nonviral nucleotides to the 3' terminus of transcripts with no 3'-terminal (A) residues eliminated infectivity. Heterogenous-length poly(A) tails, indistinguishable from the wild type, were synthesized de novo in plants inoculated with transcripts with no 3'-terminal (A) residues, demonstrating the presence of a poly(A) polymerase activity in WCIMV-infected tissue. Mutation of a putative polyadenylation motif found in the 3' noncoding region of WCIMV decreased the efficiency of polyadenylation of the progeny of transcripts with 10 3'-terminal (A) residues. The same mutation in transcripts with no 3'-terminal (A) residues abolished infectivity.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Viruses
/
Poly A
/
RNA Viruses
/
RNA, Viral
Language:
En
Journal:
Virology
Year:
1991
Type:
Article
Affiliation country:
New Zealand