Introduction of the herpes simplex virus thymidine kinase gene into mouse cells using virus DNA or transformed cell DNA.
Cell
; 13(3): 581-7, 1978 Mar.
Article
in En
| MEDLINE
| ID: mdl-207433
ABSTRACT
Cells lacking the enzyme thymidine kinase (LMTK- cells) have been transformed to a kinase-positive phenotype using sheared herpes simplex virus (HSV) DNA, and the enzyme found in these transformed cells is HSV-specific. One of the cell lines is able to complement the functional defect found in two temperature-sensitive mutants of HSV 1, and reversion of the cells to a thymidine kinase-negative phenotype results in the loss of this capability. The HSV thymidine kinase gene can also be introduced into LMTK- cells using DNA extracted from transformed cells, and the high efficiency of this procedure suggests that the state of the virus DNA in transformed cells is different from that of DNA in virus particles.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thymidine Kinase
/
DNA
/
DNA, Viral
/
Cell Transformation, Viral
/
Simplexvirus
/
Genes, Viral
Language:
En
Journal:
Cell
Year:
1978
Type:
Article