EBP1 and DRBP76/NF90 binding proteins are included in the major histocompatibility complex class II RNA operon.
Nucleic Acids Res
; 39(16): 7263-75, 2011 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-21624892
ABSTRACT
Major histocompatibility complex class II mRNAs encode heterodimeric proteins involved in the presentation of exogenous antigens during an immune response. Their 3'UTRs bind a protein complex in which we identified two factors EBP1, an ErbB3 receptor-binding protein and DRBP76, a double-stranded RNA binding nuclear protein, also known as nuclear factor 90 (NF90). Both are well-characterized regulatory factors of several mRNA molecules processing. Using either EBP1 or DRBP76/NF90-specific knockdown experiments, we established that the two proteins play a role in regulating the expression of HLA-DRA, HLA-DRB1 and HLA-DQA1 mRNAs levels. Our study represents the first indication of the existence of a functional unit that includes different transcripts involved in the adaptive immune response. We propose that the concept of 'RNA operon' may be suitable for our system in which MHCII mRNAs are modulated via interaction of their 3'UTR with same proteins.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Histocompatibility Antigens Class II
/
RNA-Binding Proteins
/
3' Untranslated Regions
/
Adaptor Proteins, Signal Transducing
/
Nuclear Factor 90 Proteins
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Nucleic Acids Res
Year:
2011
Type:
Article
Affiliation country:
Italy