A rapid and robust method for selective isotope labeling of proteins.

Lin, Myat T; Sperling, Lindsay J; Frericks Schmidt, Heather L; Tang, Ming; Samoilova, Rimma I; Kumasaka, Takashi; Iwasaki, Toshio; Dikanov, Sergei A; Rienstra, Chad M; Gennis, Robert B

Lin, Myat T; Sperling, Lindsay J; Frericks Schmidt, Heather L; Tang, Ming; Samoilova, Rimma I; Kumasaka, Takashi; Iwasaki, Toshio; Dikanov, Sergei A; Rienstra, Chad M; Gennis, Robert B.

Affiliation

Lin MT; Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.

Methods ; 55(4): 370-8, 2011 Dec.

Article in En | MEDLINE | ID: mdl-21925267

Subject(s)

Electron Transport Complex IV/biosynthesis; Escherichia coli Proteins/biosynthesis; Ferredoxins/biosynthesis; Isotope Labeling/methods; Amino Acid Motifs; Binding Sites; Electron Spin Resonance Spectroscopy; Electron Transport Complex IV/chemistry; Escherichia coli/genetics; Escherichia coli Proteins/chemistry; Ferredoxins/chemistry; Hydrogen Bonding; Iron/chemistry; Membrane Proteins/biosynthesis; Membrane Proteins/chemistry; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Organisms, Genetically Modified; Oxidation-Reduction; Protein Subunits/biosynthesis; Protein Subunits/chemistry; Recombinant Proteins/biosynthesis; Recombinant Proteins/chemistry; Spectroscopy, Fourier Transform Infrared; Sulfur/chemistry

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Electron Transport Complex IV / Escherichia coli Proteins / Ferredoxins / Isotope Labeling Language: En Journal: Methods Journal subject: BIOQUIMICA Year: 2011 Type: Article Affiliation country: United States

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