Breast Neoplasms/enzymology; Cell Membrane/metabolism; Oncogene Proteins/metabolism; Protein Serine-Threonine Kinases/metabolism; Proto-Oncogene Proteins c-akt/metabolism; Receptors, Transforming Growth Factor beta/metabolism; Ubiquitin Thiolesterase/metabolism; Animals; Breast Neoplasms/genetics; Breast Neoplasms/pathology; Cell Movement; Enzyme Stability; Epithelial-Mesenchymal Transition; Female; Gene Expression Regulation; HEK293 Cells; HeLa Cells; Humans; Mice; Mice, Knockout; Mutation; Neoplasm Invasiveness; Oncogene Proteins/deficiency; Oncogene Proteins/genetics; Phosphorylation; Protein Kinase Inhibitors/pharmacology; Protein Serine-Threonine Kinases/antagonists & inhibitors; Protein Serine-Threonine Kinases/genetics; Protein Transport; Proto-Oncogene Proteins; RNA Interference; Receptor, Transforming Growth Factor-beta Type I; Receptors, Transforming Growth Factor beta/antagonists & inhibitors; Receptors, Transforming Growth Factor beta/genetics; Signal Transduction; Time Factors; Transfection; Transforming Growth Factor beta/metabolism; Ubiquitin Thiolesterase/genetics; Ubiquitin-Specific Proteases; Ubiquitination; Zebrafish/embryology