Solubilisation of the armadillo-repeat protein ß-catenin using a zwitterionic detergent allows resolution of phosphorylated forms by 2DE.

Layton, Meredith J; Church, Nicole L; Faux, Maree C; Ji, Hong; Goode, Robert J A; Kapp, Eugene A; Burgess, Antony W; Simpson, Richard J

Layton, Meredith J; Church, Nicole L; Faux, Maree C; Ji, Hong; Goode, Robert J A; Kapp, Eugene A; Burgess, Antony W; Simpson, Richard J.

Affiliation

Layton MJ; The Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Parkville, Australia.

Electrophoresis ; 33(12): 1804-13, 2012 Jul.

Article in En | MEDLINE | ID: mdl-22740469

Subject(s)

Betaine/analogs & derivatives; beta Catenin/chemistry; Amino Acid Sequence; Animals; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/chemistry; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/metabolism; Betaine/chemistry; Caco-2 Cells; Electrophoresis, Gel, Two-Dimensional; Humans; Isoelectric Focusing; L Cells; Mice; Molecular Sequence Data; Phosphorylation; Protein Processing, Post-Translational; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Solubility; Transcription Factor 4; Transcription Factors/chemistry; Transcription Factors/metabolism; Wnt Signaling Pathway; beta Catenin/metabolism

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Betaine / Beta Catenin Limits: Animals / Humans Language: En Journal: Electrophoresis Year: 2012 Type: Article Affiliation country: Australia

Fulltext

XML

PubMed Links

Search on Google