Potentiation of hemostimulating effects of erythropoietin with pegylated hyaluronate-endo-ß-N-acetylhexosaminidase.

Dygai, A M; Zyuz'kov, G N; Zhdanov, V V; Udut, E V; Gurto, R V; Miroshnichenko, L A; Simanina, E V; Khrichkova, T Iu; Stavrova, L A; Chaikovsky, A V; Artamonov, A V; Bekarev, A A; Madonov, P G; Minakova, M Iu; Markova, T S

Dygai, A M; Zyuz'kov, G N; Zhdanov, V V; Udut, E V; Gurto, R V; Miroshnichenko, L A; Simanina, E V; Khrichkova, T Iu; Stavrova, L A; Chaikovsky, A V; Artamonov, A V; Bekarev, A A; Madonov, P G; Minakova, M Iu; Markova, T S.

Affiliation

Dygai AM; Siberian Division of the Russian Academy of Medical Sciences, Institute of Pharmacology, Tomsk, Russia.

Bull Exp Biol Med ; 153(5): 684-8, 2012 Sep.

Article in En | MEDLINE | ID: mdl-23113258

ABSTRACT

ABSTRACT

Pegylated hyaluronate-endo-ß-N-acetylhexosaminidase considerably potentiates the hemostimulating effects of erythropoietin due to intensification of proliferation and differentiation of erythroid precursors against the background of enhanced secretion of hemopoietins by nonadherent hemopoiesis-inducing environment cells and elevation of serum erythropoietin concentration. The use of the enzyme allows 10-fold reduction of the maximum effective erythropoietin dose.

Subject(s)

Anemia/drug therapy; Erythropoietin/metabolism; Erythropoietin/pharmacology; Polyethylene Glycols/metabolism; beta-N-Acetylhexosaminidases/metabolism; Animals; Carboplatin; Dose-Response Relationship, Drug; Drug Synergism; Enzyme-Linked Immunosorbent Assay; Erythrocyte Count; Erythropoietin/blood; Female; Hemoglobins/metabolism; Male; Mice; Mice, Inbred CBA; Nanotechnology/methods; Recombinant Proteins; Statistics, Nonparametric

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Collection: 01-internacional Database: MEDLINE Main subject: Polyethylene Glycols / Beta-N-Acetylhexosaminidases / Erythropoietin / Anemia Limits: Animals Language: En Journal: Bull Exp Biol Med Year: 2012 Type: Article Affiliation country: RUSSIA

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