Virus-specific DNA sequences present in cells which carry the herpes simplex virus thymidine kinase gene.
J Gen Virol
; 45(2): 489-96, 1979 Nov.
Article
in En
| MEDLINE
| ID: mdl-232133
ABSTRACT
Two independently derived cell lines which carry the herpes simplex type 2 thymidine kinase gene have been examined for the presence of HSV-2-specific DNA sequences. Both cell lines contained 1 to 3 copies per cell of a sequence lying within map co-ordinates 0.2 to 0.4 of the HSV-2 genome. Revertant cells, which contained no detectable thymidine kinase, did not contain this DNA sequence. The failure of EcoR1-restricted HSV-2 DNA to act as a donor of the thymidine kinase gene in transformation experiments suggests that the gene lies close to the EcoR1 restriction site within this sequence at a map position of approx. 0.3. The HSV-2 kinase gene is therefore approximately co-linear with the HSV-1 gene.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA, Neoplasm
/
DNA, Viral
/
Cell Transformation, Neoplastic
/
Cell Transformation, Viral
/
Simplexvirus
Limits:
Animals
Language:
En
Journal:
J Gen Virol
Year:
1979
Type:
Article