Simultaneous analysis of guaiacol and vanillin in a vanilla extract by using high-performance liquid chromatography with electrochemical detection.
Biosci Biotechnol Biochem
; 77(3): 595-600, 2013.
Article
in En
| MEDLINE
| ID: mdl-23470765
ABSTRACT
We developed and validated a new high-performance liquid chromatographic analysis for electrochemically detecting guaiacol and vanillin as important components in vanilla extract. Separation was achieved with Capcell Pak C-18 MG, the potential of the working electrode being set at +1000 mV. The respective calibration curves for guaiacol and vanillin were linear in the range of 1.60-460 µg/L and 5.90-1180 µg/L. The respective limits for the quantities of guaiacol and vanillin were 1.60 µg/L and 2.36 µg/L. The related standard deviations of the intra- and inter-day precision of the retention time and peak area were all less than 4%. The recovery of guaiacol and vanillin was both more than 97%, all of the validation data being within an acceptable range. This analysis method is well suited for the simultaneous and convenient analysis of guaiacol and vanillin in a vanilla extract to evaluate the quality of the vanilla extract.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Benzaldehydes
/
Plant Extracts
/
Vanilla
/
Guaiacol
Type of study:
Diagnostic_studies
Limits:
Female
/
Humans
/
Male
Language:
En
Journal:
Biosci Biotechnol Biochem
Journal subject:
BIOQUIMICA
/
BIOTECNOLOGIA
Year:
2013
Type:
Article
Affiliation country:
Japan