A single-stranded junction modulates nanosecond motional ordering of the substrate recognition duplex of a group I ribozyme.
Chembiochem
; 14(14): 1720-3, 2013 Sep 23.
Article
in En
| MEDLINE
| ID: mdl-23900919
ABSTRACT
Rigid spinning Site-directed spin-labeling studies using a rigid nitroxide spin label (Ç) reveal that both length and sequence of a single-stranded junction (J1/2) modulate nanosecond motional ordering of the substrate-recognition duplex (P1) of the 120 kD group I ribozyme. The studies demonstrate an approach for experimental measurements of nanosecond dynamics in high-molecular-weight RNA complexes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Catalytic
Language:
En
Journal:
Chembiochem
Journal subject:
BIOQUIMICA
Year:
2013
Type:
Article