Calcium involvement in the muscarinic response of the gastric parietal cell.
Cell Signal
; 2(2): 177-86, 1990.
Article
in En
| MEDLINE
| ID: mdl-2400633
ABSTRACT
The influence of extracellular Ca2+ on the mediation of carbachol stimulation in isolated rabbit gastric parietal cells was studied. Removing Ca2+ from extracellular medium caused a 42% decrease of the aminopyrine accumulation due to carbachol with the same EC50 value (approximately 5 microM). A short time depletion in extracellular calcium suppressed the carbachol-dependent Ca2+ influx without affecting Ca2+ release from internal stores (fura-2 measurements). Similarly, the production of inositol phosphates under cholinergic stimulation was reduced by 29%. A rapid increase in Ins(1,4,5)P3 was obtained 5 s after carbachol stimulation, and this increase was not changed in Ca2(+)-depleted medium. In contrast, a 20 min incubation with carbachol caused a 50% reduction in both basal and carbachol-stimulated inositol phosphate accumulations. In conclusion, phospholipase C activation, intracellular Ca2+ release and aminopyrine accumulation were sequentially observed following carbachol stimulation of the isolated gastric parietal cell and extracellular calcium contributed to sustain this acid secretory response.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Parietal Cells, Gastric
/
Carbachol
/
Calcium
/
Receptors, Muscarinic
Limits:
Animals
Language:
En
Journal:
Cell Signal
Year:
1990
Type:
Article
Affiliation country:
France