Fully automated analysis of chemically induced Î³H2AX foci in human peripheral blood mononuclear cells by indirect immunofluorescence.

Willitzki, Annika; Lorenz, Sebastian; Hiemann, Rico; Guttek, Karina; Goihl, Alexander; Hartig, Roland; Conrad, Karsten; Feist, Eugen; Sack, Ulrich; Schierack, Peter; Heiserich, Lisa; Eberle, Caroline; Peters, Vanessa; Roggenbuck, Dirk; Reinhold, Dirk

Willitzki, Annika; Lorenz, Sebastian; Hiemann, Rico; Guttek, Karina; Goihl, Alexander; Hartig, Roland; Conrad, Karsten; Feist, Eugen; Sack, Ulrich; Schierack, Peter; Heiserich, Lisa; Eberle, Caroline; Peters, Vanessa; Roggenbuck, Dirk; Reinhold, Dirk.

Affiliation

Willitzki A; Institute of Molecular and Clinical Immunology, Otto-von-Guericke-University, Magdeburg, Germany.

Cytometry A ; 83(11): 1017-26, 2013 Nov.

Article in En | MEDLINE | ID: mdl-24009179

Subject(s)

Fluorescent Antibody Technique, Indirect; Histones/isolation & purification; Leukocytes, Mononuclear/drug effects; DNA Breaks, Double-Stranded/drug effects; DNA Damage/drug effects; DNA Damage/genetics; Etoposide/pharmacology; Histones/blood; Humans; Leukocytes, Mononuclear/immunology

Key words

DNA double-strand breaks; automated microscopy; etoposide; human PBMCs; image analysis; Î³H2AX foci

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Leukocytes, Mononuclear / Histones / Fluorescent Antibody Technique, Indirect Type of study: Guideline Limits: Humans Language: En Journal: Cytometry A Year: 2013 Type: Article Affiliation country: Germany

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