Characterization of CpdC, a large-ring lactone-hydrolyzing enzyme from Pseudomonas sp. strain HI-70, and its use as a fusion tag facilitating overproduction of proteins in Escherichia coli.

Xu, Yali; Grosse, Stephan; Iwaki, Hiroaki; Hasegawa, Yoshie; Lau, Peter C K

Xu, Yali; Grosse, Stephan; Iwaki, Hiroaki; Hasegawa, Yoshie; Lau, Peter C K.

Affiliation

Xu Y; Aquatic and Crop Resource Development, National Research Council Canada, Montreal, Quebec, Canada.

Appl Environ Microbiol ; 79(22): 7091-100, 2013 Nov.

Article in En | MEDLINE | ID: mdl-24038681

Subject(s)

Carboxylic Ester Hydrolases/biosynthesis; Gene Expression Regulation, Bacterial; Macrolides/metabolism; Pseudomonas/enzymology; Pseudomonas/genetics; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Half-Life; Hydrogen-Ion Concentration; Hydrolases/metabolism; Inclusion Bodies/enzymology; Molecular Sequence Data; Plasmids; Sequence Analysis, DNA; Substrate Specificity; Temperature

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pseudomonas / Carboxylic Ester Hydrolases / Gene Expression Regulation, Bacterial / Macrolides Language: En Journal: Appl Environ Microbiol Year: 2013 Type: Article Affiliation country: Canada

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