¹H, ¹5N, and ¹³C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola.
Biomol NMR Assign
; 8(2): 405-8, 2014 Oct.
Article
in En
| MEDLINE
| ID: mdl-24091893
ABSTRACT
Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73%. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20-C57), that in CP includes the ß2-strand and it is involved in GlcNAc (N-acetyl-D-glucosamine) interaction, in Pop1 is predicted to be fully disordered.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ascomycota
/
Fungal Proteins
/
Nuclear Magnetic Resonance, Biomolecular
Language:
En
Journal:
Biomol NMR Assign
Journal subject:
BIOLOGIA MOLECULAR
/
MEDICINA NUCLEAR
Year:
2014
Type:
Article
Affiliation country:
Italy