Cleavage of pro-tumor necrosis factor alpha by ADAM metallopeptidase domain 17: a fluorescence-based protease assay cleaves its natural protein substrate.

Zhang, Chengquan; Zheng, Li; Nurnberg, John; Vacari, Binetti M; Zhou, Jianzhong; Wang, Yi

Zhang, Chengquan; Zheng, Li; Nurnberg, John; Vacari, Binetti M; Zhou, Jianzhong; Wang, Yi.

Affiliation

Zhang C; EZBiolab, Carmel, IN 46032, USA.

Anal Biochem ; 445: 14-9, 2014 Jan 15.

Article in En | MEDLINE | ID: mdl-24096196

Subject(s)

ADAM Proteins/metabolism; Enzyme Assays; Fluorescein-5-isothiocyanate/chemistry; Protein Precursors/metabolism; Tumor Necrosis Factor-alpha/metabolism; ADAM Proteins/chemistry; ADAM17 Protein; Amino Acid Sequence; Fluorescein-5-isothiocyanate/metabolism; Hydrogen-Ion Concentration; Molecular Sequence Data; Peptides/chemistry; Peptides/metabolism; Protein Binding; Protein Precursors/antagonists & inhibitors; Protein Precursors/genetics; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Substrate Specificity; Tumor Necrosis Factor-alpha/antagonists & inhibitors; Tumor Necrosis Factor-alpha/genetics

Key words

Adam 17; Protease; Protease assay; TACE; TNFα

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Precursors / Tumor Necrosis Factor-alpha / Fluorescein-5-isothiocyanate / ADAM Proteins / Enzyme Assays Type of study: Prognostic_studies Language: En Journal: Anal Biochem Year: 2014 Type: Article Affiliation country: United States

Fulltext

XML

PubMed Links

Search on Google