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Use of ethanol extract of Mycobacterium bovis for detection of specific antibodies in sera of farmed red deer (Cervus elaphus) with bovine tuberculosis.
Wadhwa, Ashutosh; Johnson, Rachel E; Mackintosh, Colin G; Griffin, J Frank T; Waters, W Ray; Bannantine, John P; Eda, Shigetoshi.
Affiliation
  • Eda S; Department of Forestry, Wildlife and Fisheries, Center for Wildlife Health, University of Tennessee Institute of Agriculture, Knoxville, Tennessee 37996, USA. seda@utk.edu.
BMC Vet Res ; 9: 256, 2013 Dec 17.
Article in En | MEDLINE | ID: mdl-24341485
ABSTRACT

BACKGROUND:

Bovine tuberculosis (bTB) in wildlife species poses a threat to domestic livestock in many situations. Control programs for bTB in livestock depend on testing and slaughtering the positive animals; however, the currently available diagnostic tests often have poor specificity. In our previous study, we developed a specific and sensitive enzyme linked immunosorbent assay (ELISA) for another mycobacterial disease - Johne's disease, using surface antigens of Mycobacterium avium ssp. paratuberculosis (MAP) extracted by briefly agitating the bacilli in 80% ethanol solution. The ELISA test was named ethanol vortex ELISA (EVELISA). The objective of this study is to examine whether EVELISA technique could be used to specifically detect anti-Mycobacterium bovis (M. bovis) antibodies in the serum of M. bovis-infected farmed red deer (Cervus elaphus). We tested a total of 45 red deer serum samples, divided in 3 groups - uninfected animals (n = 15), experimentally infected with M. bovis (n = 15) and experimentally infected with MAP (n = 15).

RESULTS:

The presence of anti-M. bovis antibodies was tested using an ethanol extract of M. bovis. Without absorption of anti-MAP cross reactive antibodies, it was found that 13 out of the 15 MAP-infected animals showed high antibody binding. Using heat killed MAP as an absorbent of cross reactive antibodies, anti-M. bovis antibodies were detected in 86.7% of M. bovis-infected animals with minor false positive results caused by MAP infection.

CONCLUSIONS:

The results from this study suggest that EVELISA may form a basis for a sensitive and specific test for the diagnosis of bTB in farmed red deer.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tuberculosis, Bovine / Deer / Antibodies, Bacterial / Mycobacterium bovis Type of study: Diagnostic_studies Limits: Animals Language: En Journal: BMC Vet Res Journal subject: MEDICINA VETERINARIA Year: 2013 Type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tuberculosis, Bovine / Deer / Antibodies, Bacterial / Mycobacterium bovis Type of study: Diagnostic_studies Limits: Animals Language: En Journal: BMC Vet Res Journal subject: MEDICINA VETERINARIA Year: 2013 Type: Article