A study on cryoprotectant solution suitable for vitrification of rat two-cell stage embryos.

Eto, Tomoo; Takahashi, Riichi; Kamisako, Tsutomu; Hioki, Kyoji; Sotomaru, Yusuke

Eto, Tomoo; Takahashi, Riichi; Kamisako, Tsutomu; Hioki, Kyoji; Sotomaru, Yusuke.

Affiliation

Eto T; Central Institute for Experimental Animals, Kawasaki 210-0821, Japan. Electronic address: etoh@ciea.or.jp.
Takahashi R; In-Vivo Science, Inc., Kawasaki 210-0821, Japan.
Kamisako T; Central Institute for Experimental Animals, Kawasaki 210-0821, Japan.
Hioki K; Central Institute for Experimental Animals, Kawasaki 210-0821, Japan.
Sotomaru Y; Natural Science Center for Basic Research and Development, Hiroshima University, Hiroshima, Japan.

Cryobiology ; 68(1): 147-51, 2014 Feb.

Article in En | MEDLINE | ID: mdl-24462541

Subject(s)

Cryopreservation; Cryoprotective Agents/pharmacology; Embryo, Mammalian/drug effects; Embryonic Development/drug effects; Vitrification; Animals; Cell Membrane Permeability; Cell Survival/drug effects; Dimethyl Sulfoxide/pharmacology; Embryo, Mammalian/cytology; Embryo, Mammalian/physiology; Ethylene Glycol/pharmacology; Female; Glycerol/pharmacology; Male; Osmolar Concentration; Povidone/pharmacology; Propylene Glycol/pharmacology; Rats; Silicon Dioxide/pharmacology; Sucrose/pharmacology

Key words

Cryopreservation; Cryoprotectant solution; P10; PEPeS; Rat embryos; Strain preservation; Vitrification

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cryopreservation / Cryoprotective Agents / Embryonic Development / Embryo, Mammalian / Vitrification Limits: Animals Language: En Journal: Cryobiology Year: 2014 Type: Article

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