Genome-wide miRNA-profiling of aflatoxin B1-induced hepatic injury using deep sequencing.
Toxicol Lett
; 226(2): 140-9, 2014 Apr 21.
Article
in En
| MEDLINE
| ID: mdl-24472605
ABSTRACT
Aflatoxin B1 is a potent carcinogen which can induce** hepatocellular carcinoma (HCC) in mammals. Though microRNAs are known to play important roles in tumorigenesis, the functional complexity of microRNAs in AFB1-induced hepatocellular tumorigenesis has not yet been elucidated. Here, we applied Illumina deep sequencing technology for high-throughput profiling of microRNA in rat liver tissue before and after treatment with aflatoxin B1. Analysis of mature miRNAs from different arms of pre-miRNAs allowed us to identify the predominant form of miRNA. We studied the differential expression profile of miRNAs in two libraries, identifying several cancer-related microRNAs which exhibit abnormal expression. KEGG analysis indicated that predicted target genes of differentially expressed miRNAs are involved in cancer-related pathways. Bioinformatic analysis predicted 16 potential novel miRNAs. Our work provides new insights at the miRNA level into AFB1-induced hepatic injury which may lead to HCC.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA Precursors
/
Aflatoxin B1
/
Gene Expression Profiling
/
MicroRNAs
/
Chemical and Drug Induced Liver Injury
/
High-Throughput Nucleotide Sequencing
/
Liver
Type of study:
Etiology_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Toxicol Lett
Year:
2014
Type:
Article
Affiliation country:
China