Effect of short training on vaginal fluid microscopy (wet mount) learning.
J Low Genit Tract Dis
; 19(2): 165-9, 2015 Apr.
Article
in En
| MEDLINE
| ID: mdl-25148226
ABSTRACT
OBJECTIVE:
Is it feasible to learn the basics of wet mount microscopy of vaginal fluid in 10 hours? MATERIALS ANDMETHODS:
This is a pilot project wherein 6 students with different grades of education were invited for being tested on their ability to read wet mount microscopic slides before and after 10 hours of hands-on training. Microscopy was performed according to a standard protocol (Femicare, Tienen, Belgium). Before and after training, all students had to evaluate a different set of 50 digital slides. Different diagnoses and microscopic patterns had to be scored. κ indices were calculated compared with the expert reading.RESULTS:
All readers improved their mean scores significantly, especially for the most important types of altered flora (p < .0001). The mean increase in reading concordance (κ from 0.64 to 0.75) of 1 student with a solid previous experience with microscopy did not reach statistical significance, but the remaining 5 students all improved their scores from poor performance (all κ < 0.20) to moderate (κ = 0.53, n = 1) to good (κ > 0.61, n = 4) concordance. Reading quality improved and reached fair to good concordance on all microscopic items studied, except for the detection of parabasal cells and cytolytic flora.CONCLUSIONS:
Although further improvement is still possible, a short training course of 10 hours enables vast improvement on wet mount microscopy accuracy and results in fair to good concordance of the most important variables of the vaginal flora compared to a reference reader.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Candidiasis, Vulvovaginal
/
Vaginosis, Bacterial
/
Vaginal Discharge
/
Education, Medical, Continuing
/
Microscopy
Type of study:
Guideline
Limits:
Female
/
Humans
Country/Region as subject:
Europa
Language:
En
Journal:
J Low Genit Tract Dis
Journal subject:
GINECOLOGIA
Year:
2015
Type:
Article
Affiliation country:
Brazil