The structure of a tetrameric &#945;-carbonic anhydrase from Thermovibrio ammonificans reveals a core formed around intermolecular disulfides that contribute to its thermostability.

James, Paul; Isupov, Michail N; Sayer, Christopher; Saneei, Vahid; Berg, Svein; Lioliou, Maria; Kotlar, Hans Kristian; Littlechild, Jennifer A

The structure of a tetrameric α-carbonic anhydrase from Thermovibrio ammonificans reveals a core formed around intermolecular disulfides that contribute to its thermostability.

James, Paul; Isupov, Michail N; Sayer, Christopher; Saneei, Vahid; Berg, Svein; Lioliou, Maria; Kotlar, Hans Kristian; Littlechild, Jennifer A.

Affiliation

James P; Henry Wellcome Building for Biocatalysis, Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, England.
Isupov MN; Henry Wellcome Building for Biocatalysis, Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, England.
Sayer C; Henry Wellcome Building for Biocatalysis, Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, England.
Saneei V; Henry Wellcome Building for Biocatalysis, Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, England.
Berg S; Statoil Research Centre, Statoil, Arkitekt Ebbells veg 10, Rotvoll, 7005 Trondheim, Norway.
Lioliou M; Statoil Research Centre, Statoil, Arkitekt Ebbells veg 10, Rotvoll, 7005 Trondheim, Norway.
Kotlar HK; Statoil Research Centre, Statoil, Arkitekt Ebbells veg 10, Rotvoll, 7005 Trondheim, Norway.
Littlechild JA; Henry Wellcome Building for Biocatalysis, Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, England.

Acta Crystallogr D Biol Crystallogr ; 70(Pt 10): 2607-18, 2014 Oct.

Article in En | MEDLINE | ID: mdl-25286845

Subject(s)

Carbonic Anhydrases/chemistry; Carbonic Anhydrases/metabolism; Acetazolamide/chemistry; Bacteria/chemistry; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Carbon Dioxide/metabolism; Carbonic Anhydrase Inhibitors/chemistry; Carbonic Anhydrase Inhibitors/metabolism; Carbonic Anhydrases/genetics; Catalytic Domain; Cloning, Molecular; Crystallography, X-Ray; Disulfides/chemistry; Enzyme Stability; Kinetics; Models, Molecular; Nitrophenols/metabolism; Protein Conformation; Sulfanilamide; Sulfanilamides/chemistry; Temperature

Key words

acetazolamide; carbon dioxide capture; carbonic anhydrase; thermal stability; thermophilic

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carbonic Anhydrases Language: En Journal: Acta Crystallogr D Biol Crystallogr Year: 2014 Type: Article Affiliation country: United kingdom

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