Multiplex engineering of industrial yeast genomes using CRISPRm.

Ryan, Owen W; Cate, Jamie H D

Ryan, Owen W; Cate, Jamie H D.

Affiliation

Ryan OW; Energy Biosciences Institute, University of California, Berkeley, California, USA.
Cate JH; Energy Biosciences Institute, University of California, Berkeley, California, USA; Department of Molecular and Cell Biology, University of California, Berkeley, California, USA; Department of Chemistry, University of California, Berkeley, California, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA. Electronic address: jcate@lbl.gov.

Methods Enzymol ; 546: 473-89, 2014.

Article in En | MEDLINE | ID: mdl-25398354

Subject(s)

CRISPR-Cas Systems; Directed Molecular Evolution/methods; Genetic Engineering/methods; Industrial Microbiology/methods; Saccharomyces cerevisiae/genetics; Base Sequence; CRISPR-Associated Proteins/genetics; Clustered Regularly Interspaced Short Palindromic Repeats; Endonucleases/genetics; Gene Library; Genome, Fungal; Molecular Sequence Data; Plasmids/genetics; RNA, Guide, Kinetoplastida/genetics

Key words

CRISPRm; Cas9; Chromosomal integration; DNA libraries; Directed evolution; Homology-directed repair; Industrial yeast; Multiplex genome editing; Saccharomyces cerevisiae; Synthetic biology

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Saccharomyces cerevisiae / Industrial Microbiology / Genetic Engineering / Directed Molecular Evolution / CRISPR-Cas Systems Language: En Journal: Methods Enzymol Year: 2014 Type: Article Affiliation country: United States

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