Isolation and characterization of a proteinaceous &#945;-amylase inhibitor AAI-CC5 from Streptomyces sp. CC5, and its gene cloning and expression.

Sun, Zhibin; Lu, Weihao; Liu, Pingping; Wang, Hui; Huang, Yan; Zhao, Yuguo; Kong, Yi; Cui, Zhongli

Isolation and characterization of a proteinaceous α-amylase inhibitor AAI-CC5 from Streptomyces sp. CC5, and its gene cloning and expression.

Sun, Zhibin; Lu, Weihao; Liu, Pingping; Wang, Hui; Huang, Yan; Zhao, Yuguo; Kong, Yi; Cui, Zhongli.

Affiliation

Sun Z; Key Laboratory of Environmental Microbiology of Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

Antonie Van Leeuwenhoek ; 107(2): 345-56, 2015 Feb.

Article in En | MEDLINE | ID: mdl-25411086

Subject(s)

Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Enzyme Inhibitors/isolation & purification; Enzyme Inhibitors/metabolism; Streptomyces/metabolism; alpha-Amylases/antagonists & inhibitors; Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Chromatography, Affinity; Cloning, Molecular; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; Enzyme Inhibitors/chemistry; Escherichia coli/genetics; Hot Temperature; Inhibitory Concentration 50; Molecular Sequence Data; Molecular Weight; Protein Stability; Proteolysis; Sequence Analysis, DNA; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Streptomyces/genetics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Streptomyces / Bacterial Proteins / Enzyme Inhibitors / Alpha-Amylases Language: En Journal: Antonie Van Leeuwenhoek Year: 2015 Type: Article

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