A versatile coupled cell-free transcription-translation system based on tobacco BY-2 cell lysates.

Buntru, Matthias; Vogel, Simon; Stoff, Katrin; Spiegel, Holger; Schillberg, Stefan

Buntru, Matthias; Vogel, Simon; Stoff, Katrin; Spiegel, Holger; Schillberg, Stefan.

Affiliation

Buntru M; Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, Aachen, 52074, Germany.

Biotechnol Bioeng ; 112(5): 867-78, 2015 May.

Article in En | MEDLINE | ID: mdl-25421615

Subject(s)

Cell-Free System/metabolism; Nicotiana/cytology; Nicotiana/genetics; Protein Biosynthesis; Recombinant Proteins/genetics; Transcription, Genetic; Animals; Antibodies/genetics; Antibodies/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biotechnology/methods; Cells, Cultured; Glycosylation; Humans; Luciferases, Firefly/genetics; Luciferases, Firefly/metabolism; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Recombinant Proteins/metabolism; Nicotiana/metabolism

Key words

BY-2 cell lysate; cell-free protein synthesis; coupled transcription-translation; high-throughput screening; protein expression

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Nicotiana / Transcription, Genetic / Protein Biosynthesis / Recombinant Proteins / Cell-Free System Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Biotechnol Bioeng Year: 2015 Type: Article Affiliation country: Germany

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