Monensin, a small molecule ionophore, can be used to increase high mannose levels on monoclonal antibodies generated by Chinese hamster ovary production cell-lines.

Pande, Sandhya; Rahardjo, Ayu; Livingston, Brittney; Mujacic, Mirna

Pande, Sandhya; Rahardjo, Ayu; Livingston, Brittney; Mujacic, Mirna.

Affiliation

Pande S; Cell Sciences and Technology, Amgen, 1201 Amgen Court W, Seattle, Washington, 98119.
Rahardjo A; Cell Sciences and Technology, Amgen, 1201 Amgen Court W, Seattle, Washington, 98119.
Livingston B; Analytical Sciences, Amgen, 1201 Amgen Court W, Seattle, Washington, 98119.
Mujacic M; Cell Sciences and Technology, Amgen, 1201 Amgen Court W, Seattle, Washington, 98119. mmujacic@gmail.com.

Biotechnol Bioeng ; 112(7): 1383-94, 2015 Jul.

Article in En | MEDLINE | ID: mdl-25619381

Subject(s)

Antibodies, Monoclonal/metabolism; CHO Cells/drug effects; CHO Cells/metabolism; Ionophores/metabolism; Mannose/metabolism; Monensin/metabolism; Animals; Antibodies, Monoclonal/genetics; Cricetulus; Glycosylation; Humans; Recombinant Proteins/genetics; Recombinant Proteins/metabolism

Key words

Chinese hamster ovary cells; glycosylation; golgi; high mannose; monensin; monoclonal antibodies; product quality control

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Monensin / CHO Cells / Ionophores / Mannose / Antibodies, Monoclonal Limits: Animals / Humans Language: En Journal: Biotechnol Bioeng Year: 2015 Type: Article

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