An engineered old yellow enzyme that enables efficient synthesis of (4R,6R)-Actinol in a one-pot reduction system.

Horita, Shoichiro; Kataoka, Michihiko; Kitamura, Nahoko; Nakagawa, Takuya; Miyakawa, Takuya; Ohtsuka, Jun; Nagata, Koji; Shimizu, Sakayu; Tanokura, Masaru

Horita, Shoichiro; Kataoka, Michihiko; Kitamura, Nahoko; Nakagawa, Takuya; Miyakawa, Takuya; Ohtsuka, Jun; Nagata, Koji; Shimizu, Sakayu; Tanokura, Masaru.

Affiliation

Horita S; Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan).

Chembiochem ; 16(3): 440-5, 2015 Feb 09.

Article in En | MEDLINE | ID: mdl-25639703

Subject(s)

Cyclohexanols/chemical synthesis; Cyclohexanones/chemical synthesis; NADPH Dehydrogenase/chemistry; NADPH Dehydrogenase/metabolism; Protein Engineering/methods; Amino Acid Sequence; Benzaldehydes/chemistry; Benzaldehydes/metabolism; Biocatalysis; Candida/enzymology; Catalytic Domain; Crystallography, X-Ray; Cyclohexanones/metabolism; Fungal Proteins/chemistry; Fungal Proteins/metabolism; Hydrogen Bonding; Molecular Sequence Data; Mutation; NADPH Dehydrogenase/genetics; Oxidation-Reduction; Protein Conformation; Stereoisomerism; Substrate Specificity

Key words

X-ray crystallography; biocatalysis; enzyme catalysis; enzymes; old yellow enzyme

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Engineering / Cyclohexanols / Cyclohexanones / NADPH Dehydrogenase Language: En Journal: Chembiochem Journal subject: BIOQUIMICA Year: 2015 Type: Article

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