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Selective modulation of the functions of a conserved DNA motor by a histone fold complex.
Xue, Xiaoyu; Choi, Koyi; Bonner, Jacob N; Szakal, Barnabas; Chen, Yu-Hung; Papusha, Alma; Saro, Dorina; Niu, Hengyao; Ira, Grzegorz; Branzei, Dana; Sung, Patrick; Zhao, Xiaolan.
Affiliation
  • Xue X; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA;
  • Choi K; Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA; Programs in Biochemistry, Cell, and Molecular Biology, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10065, USA;
  • Bonner JN; Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA; Programs in Biochemistry, Cell, and Molecular Biology, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10065, USA;
  • Szakal B; IFOM, Fondazione Istituto FIRC di Oncologia Molecolare, Milan 20139, Italy;
  • Chen YH; Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA; Programs in Biochemistry, Cell, and Molecular Biology, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10065, USA;
  • Papusha A; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
  • Saro D; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA;
  • Niu H; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA;
  • Ira G; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
  • Branzei D; IFOM, Fondazione Istituto FIRC di Oncologia Molecolare, Milan 20139, Italy;
  • Sung P; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA;
  • Zhao X; Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA; Programs in Biochemistry, Cell, and Molecular Biology, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10065, USA; zhaox1@mskcc.org.
Genes Dev ; 29(10): 1000-5, 2015 05 15.
Article in En | MEDLINE | ID: mdl-25956905
ABSTRACT
Budding yeast Mph1 helicase and its orthologs drive multiple DNA transactions. Elucidating the mechanisms that regulate these motor proteins is central to understanding genome maintenance processes. Here, we show that the conserved histone fold MHF complex promotes Mph1-mediated repair of damaged replication forks but does not influence the outcome of DNA double-strand break repair. Mechanistically, scMHF relieves the inhibition imposed by the structural maintenance of chromosome protein Smc5 on Mph1 activities relevant to replication-associated repair through binding to Mph1 but not DNA. Thus, scMHF is a function-specific enhancer of Mph1 that enables flexible response to different genome repair situations.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Saccharomyces cerevisiae / DNA / Histones / Saccharomyces cerevisiae Proteins / DNA-Binding Proteins Language: En Journal: Genes Dev Journal subject: BIOLOGIA MOLECULAR Year: 2015 Type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Saccharomyces cerevisiae / DNA / Histones / Saccharomyces cerevisiae Proteins / DNA-Binding Proteins Language: En Journal: Genes Dev Journal subject: BIOLOGIA MOLECULAR Year: 2015 Type: Article