Development of a general-purpose method for cell purification using Cre/loxP-mediated recombination.

Kuroki, Shunsuke; Akiyoshi, Mika; Ideguchi, Ko; Kitano, Satsuki; Miyachi, Hitoshi; Hirose, Michiko; Mise, Nathan; Abe, Kuniya; Ogura, Atsuo; Tachibana, Makoto

Kuroki, Shunsuke; Akiyoshi, Mika; Ideguchi, Ko; Kitano, Satsuki; Miyachi, Hitoshi; Hirose, Michiko; Mise, Nathan; Abe, Kuniya; Ogura, Atsuo; Tachibana, Makoto.

Affiliation

Kuroki S; Department of Enzyme Chemistry, Institute for Enzyme Research, Tokushima University, Tokushima, Japan.
Akiyoshi M; Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Sakyo-Ku, Kyoto, Japan.
Ideguchi K; Department of Enzyme Chemistry, Institute for Enzyme Research, Tokushima University, Tokushima, Japan.
Kitano S; Graduate School of Biostudies, Kyoto University, Sakyo-Ku, Kyoto, Japan.
Miyachi H; Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Sakyo-Ku, Kyoto, Japan.
Hirose M; Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Sakyo-Ku, Kyoto, Japan.
Mise N; Bioresource Center, RIKEN Tsukuba Institute, Tsukuba, Japan.
Abe K; Bioresource Center, RIKEN Tsukuba Institute, Tsukuba, Japan.
Ogura A; Bioresource Center, RIKEN Tsukuba Institute, Tsukuba, Japan.
Tachibana M; Bioresource Center, RIKEN Tsukuba Institute, Tsukuba, Japan.

Genesis ; 53(6): 387-93, 2015 Jun.

Article in En | MEDLINE | ID: mdl-26012873

Subject(s)

Embryo, Mammalian/cytology; Embryo, Mammalian/metabolism; Recombination, Genetic; Sertoli Cells/metabolism; Animals; Animals, Newborn; Cells, Cultured; Embryo, Mammalian/embryology; Female; Flow Cytometry; Gene Knockout Techniques; Humans; Immunohistochemistry; Integrases/genetics; Integrases/metabolism; Male; Mice, Transgenic; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; RNA, Untranslated/genetics; Receptors, Nerve Growth Factor/genetics; Receptors, Nerve Growth Factor/metabolism; Steroidogenic Factor 1/genetics; Steroidogenic Factor 1/metabolism

Key words

Cre/loxP-mediated recombination; hCD271; immunological cell purification; mouse

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombination, Genetic / Sertoli Cells / Embryo, Mammalian Limits: Animals / Female / Humans / Male Language: En Journal: Genesis Journal subject: BIOLOGIA MOLECULAR Year: 2015 Type: Article Affiliation country: Japan

Fulltext

XML

PubMed Links

Search on Google