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Combined metabolomics and proteomics reveals hypoxia as a cause of lower productivity on scale-up to a 5000-liter CHO bioprocess.
Gao, Yuanwei; Ray, Somak; Dai, Shujia; Ivanov, Alexander R; Abu-Absi, Nicholas R; Lewis, Amanda M; Huang, Zhuangrong; Xing, Zizhuo; Borys, Michael C; Li, Zheng Jian; Karger, Barry L.
Affiliation
  • Gao Y; Barnett Institute and Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA.
  • Ray S; Barnett Institute and Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA.
  • Dai S; Barnett Institute and Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA.
  • Ivanov AR; Barnett Institute and Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA.
  • Abu-Absi NR; Biologics Development, Global Manufacturing and Supply, Bristol-Myers Squibb, 38 Jackson Road, Devens, MA, USA.
  • Lewis AM; Biologics Development, Global Manufacturing and Supply, Bristol-Myers Squibb, 38 Jackson Road, Devens, MA, USA.
  • Huang Z; Biologics Development, Global Manufacturing and Supply, Bristol-Myers Squibb, 38 Jackson Road, Devens, MA, USA.
  • Xing Z; Biologics Development, Global Manufacturing and Supply, Bristol-Myers Squibb, 38 Jackson Road, Devens, MA, USA.
  • Borys MC; Biologics Development, Global Manufacturing and Supply, Bristol-Myers Squibb, 38 Jackson Road, Devens, MA, USA.
  • Li ZJ; Biologics Development, Global Manufacturing and Supply, Bristol-Myers Squibb, 38 Jackson Road, Devens, MA, USA.
  • Karger BL; Barnett Institute and Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA. b.karger@neu.edu.
Biotechnol J ; 11(9): 1190-200, 2016 Sep.
Article in En | MEDLINE | ID: mdl-27213298
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fibronectins / Reactive Oxygen Species / Proteomics / Metabolomics / Batch Cell Culture Techniques Limits: Animals / Humans Language: En Journal: Biotechnol J Journal subject: BIOTECNOLOGIA Year: 2016 Type: Article Affiliation country: United States
Fulltext
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fibronectins / Reactive Oxygen Species / Proteomics / Metabolomics / Batch Cell Culture Techniques Limits: Animals / Humans Language: En Journal: Biotechnol J Journal subject: BIOTECNOLOGIA Year: 2016 Type: Article Affiliation country: United States