Conformational flexibility related to enzyme activity: evidence for a dynamic active-site gatekeeper function of Tyr(215) in Aerococcus viridans lactate oxidase.

Stoisser, Thomas; Brunsteiner, Michael; Wilson, David K; Nidetzky, Bernd

Stoisser, Thomas; Brunsteiner, Michael; Wilson, David K; Nidetzky, Bernd.

Affiliation

Stoisser T; Research Center Pharmaceutical Engineering, Inffeldgasse 13, A-8010 Graz, Austria.
Brunsteiner M; Graz University of Technology, Institute of Biotechnology and Biochemical Engineering, NAWI Graz, Petersgasse 12, A-8010 Graz, Austria.
Wilson DK; Research Center Pharmaceutical Engineering, Inffeldgasse 13, A-8010 Graz, Austria.
Nidetzky B; Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA.

Sci Rep ; 6: 27892, 2016 06 15.

Article in En | MEDLINE | ID: mdl-27302031

Subject(s)

Aerococcus/enzymology; Mixed Function Oxygenases/chemistry; Mixed Function Oxygenases/metabolism; Tyrosine/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Catalytic Domain; Crystallography, X-Ray; Hydrogen Bonding; Hydrogen-Ion Concentration; Kinetics; Lactic Acid/metabolism; Mixed Function Oxygenases/genetics; Molecular Dynamics Simulation; Mutation; Phenylalanine/chemistry; Phenylalanine/metabolism; Protein Conformation; Pyruvic Acid/chemistry; Pyruvic Acid/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tyrosine / Aerococcus / Mixed Function Oxygenases Language: En Journal: Sci Rep Year: 2016 Type: Article Affiliation country: Austria

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