TCR-contacting residues orientation and HLA-DRß* binding preference determine long-lasting protective immunity against malaria.
Biochem Biophys Res Commun
; 477(4): 654-660, 2016 09 02.
Article
in En
| MEDLINE
| ID: mdl-27363338
ABSTRACT
Fully-protective, long-lasting, immunological (FPLLI) memory against Plasmodium falciparum malaria regarding immune protection-inducing protein structures (IMPIPS) vaccinated into monkeys previously challenged and re-challenged 60 days later with a lethal Aotus monkey-adapted P. falciparum strain was found to be associated with preferential high binding capacity to HLA-DRß1* allelic molecules of the major histocompatibility class II (MHC-II), rather than HLA-DRß3*, ß4*, ß5* alleles. Complete PPIIL 3D structure, a longer distance (26.5 Å ± 1.5 Å) between residues perfectly fitting into HLA-DRß1*PBR pockets 1 and 9, a gauche(-) rotamer orientation in p8 TCR-contacting polar residue and a larger volume of polar p2 residues was also found. This data, in association with previously-described p3 and p7 apolar residues having gauche(+) orientation to form a perfect MHC-II-peptide-TCR complex, determines the stereo-electronic and topochemical characteristics associated with FPLLI immunological memory.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmodium falciparum
/
Receptors, Antigen, T-Cell
/
HLA-DR beta-Chains
/
Malaria
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2016
Type:
Article
Affiliation country:
Colombia