Methionine sulfoximine supplementation enhances productivity in GS-CHOK1SV cell lines through glutathione biosynthesis.

Feary, Marc; Racher, Andrew J; Young, Robert J; Smales, C Mark

Feary, Marc; Racher, Andrew J; Young, Robert J; Smales, C Mark.

Affiliation

Feary M; New Expression Technologies Group, Research and Technology, Lonza Biologics, Granta Park, Cambridge, CB21 6GS, U.K.
Racher AJ; Future Technologies, Research and Technology, Lonza Biologics, Slough, SL1 4DX, U.K.
Young RJ; New Expression Technologies Group, Research and Technology, Lonza Biologics, Granta Park, Cambridge, CB21 6GS, U.K.
Smales CM; Centre for Industrial Biotechnology and School of Biosciences, University of Kent, Canterbury, CT2 7NJ, U.K.

Biotechnol Prog ; 33(1): 17-25, 2017 01.

Article in En | MEDLINE | ID: mdl-27689785

Subject(s)

Antibodies, Monoclonal/biosynthesis; Cell Culture Techniques/methods; Glutamate-Ammonia Ligase/metabolism; Glutathione/biosynthesis; Recombinant Proteins/biosynthesis; Animals; Batch Cell Culture Techniques/methods; Buthionine Sulfoximine/chemistry; CHO Cells; Cricetinae; Cricetulus; Culture Media/chemistry; Glutamine/chemistry; Methionine Sulfoximine/metabolism; Transfection

Key words

CHO cells; bioprocess development; buthionine sulfoximine; gene expression systems; glutamine synthetase; glutathione; industrial biotechnology; methionine sulfoximine

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Proteins / Cell Culture Techniques / Glutamate-Ammonia Ligase / Glutathione / Antibodies, Monoclonal Limits: Animals Language: En Journal: Biotechnol Prog Journal subject: BIOTECNOLOGIA Year: 2017 Type: Article Affiliation country: United kingdom

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