The Use of Laser Microirradiation to Investigate the Roles of Cohesins in DNA Repair.
Methods Mol Biol
; 1515: 227-242, 2017.
Article
in En
| MEDLINE
| ID: mdl-27797083
ABSTRACT
In addition to their mitotic and transcriptional functions, cohesin plays critical roles in DNA damage response (DDR) and repair. Specifically, cohesin promotes homologous recombination (HR) repair of DNA double-strand breaks (DSBs), which is conserved from yeast to humans, and is a critical effector of ATM/ATR DDR kinase-mediated checkpoint control in mammalian cells. Optical laser microirradiation has been instrumental in revealing the damage site-specific functions of cohesin and, more recently, uncovering the unique role of cohesin-SA2, one of the two cohesin complexes uniquely present in higher eukaryotes, in DNA repair in human cells. In this review, we briefly describe what we know about cohesin function and regulation in response to DNA damage, and discuss the optimized laser microirradiation conditions used to analyze cohesin responses to DNA damage in vivo.
Key words
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nuclear Proteins
/
Chromosomal Proteins, Non-Histone
/
Cell Cycle
/
Chromatids
/
Cell Cycle Proteins
/
Molecular Biology
Limits:
Animals
/
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2017
Type:
Article
Affiliation country:
United States