[Expression of vasoactive intestinal peptide in peripheral blood of children with hand, foot and mouth disease].
Zhongguo Dang Dai Er Ke Za Zhi
; 18(11): 1106-1110, 2016 Nov.
Article
in Zh
| MEDLINE
| ID: mdl-27817774
ABSTRACT
OBJECTIVE:
To investigate the expression of vasoactive intestinal peptide (VIP) in peripheral blood of children with hand, foot and mouth disease and its significance.METHODS:
According to the condition of the disease, 86 children with hand, foot and mouth disease were classified into phase 1 group (19 children) and phase 2 group (67 children). ELISA was used to measure the concentrations of plasma VIP, interferon-γ (IFN-γ), and interleukin-4 (IL-4) in peripheral blood. Flow cytometry was used to measure CD3+, CD4+, and CD8+ T lymphocyte subsets. RT-PCR was used for qualitative detection of enterovirus 71 (EV71) RNA in stool.RESULTS:
Compared with the phase 1 group, the phase 2 group had a significantly higher positive rate of EV71-RNA (P<0.05) and significantly higher serum levels of IgG, IgA, IgM, and C3 (P<0.05). The phase 2 group had significantly lower proportions of peripheral CD3+, CD4+, and CD8+ T lymphocyte subsets than the phase 1 group (P<0.05), as well as significantly lower proportion of peripheral B cells and CD4+/CD8+ ratio than the phase 1 group (P<0.05). The phase 2 group also had a significantly lower concentration of VIP in peripheral blood than the phase 1 group (P<0.05). In the 86 children with hand, foot and mouth disease, the concentration of VIP in peripheral blood was positively correlated with the proportion of CD4+ T lymphocyte subset and CD4+/CD8+ ratio (r=0.533 and 0.532 respectively; P<0.05).CONCLUSIONS:
VIP may be an important marker of the severity of hand, foot and mouth disease.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vasoactive Intestinal Peptide
/
Hand, Foot and Mouth Disease
Type of study:
Qualitative_research
Limits:
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
Language:
Zh
Journal:
Zhongguo Dang Dai Er Ke Za Zhi
Year:
2016
Type:
Article
Affiliation country:
China