The T296V Mutant of Amorpha-4,11-diene Synthase Is Defective in Allylic Diphosphate Isomerization but Retains the Ability To Cyclize the Intermediate (3R)-Nerolidyl Diphosphate to Amorpha-4,11-diene.

Li, Zhenqiu; Gao, Ruiping; Hao, Qinggang; Zhao, Huifang; Cheng, Longbin; He, Fang; Liu, Li; Liu, Xiuhua; Chou, Wayne K W; Zhu, Huajie; Cane, David E

Li, Zhenqiu; Gao, Ruiping; Hao, Qinggang; Zhao, Huifang; Cheng, Longbin; He, Fang; Liu, Li; Liu, Xiuhua; Chou, Wayne K W; Zhu, Huajie; Cane, David E.

Affiliation

Chou WK; Department of Chemistry, Brown University , Box H, Providence, Rhode Island 02912-9108, United States.
Cane DE; Department of Chemistry, Brown University , Box H, Providence, Rhode Island 02912-9108, United States.

Biochemistry ; 55(48): 6599-6604, 2016 Dec 06.

Article in En | MEDLINE | ID: mdl-27933789

Subject(s)

Alkyl and Aryl Transferases/chemistry; Diphosphates/chemistry; Mutation, Missense; Plant Proteins/chemistry; Polyisoprenyl Phosphates/chemistry; Sesquiterpenes/chemistry; Alkyl and Aryl Transferases/genetics; Alkyl and Aryl Transferases/metabolism; Artemisia annua/enzymology; Artemisia annua/genetics; Artemisia annua/metabolism; Biocatalysis; Cyclization; Diphosphates/metabolism; Gas Chromatography-Mass Spectrometry; Kinetics; Models, Chemical; Molecular Structure; Plant Proteins/genetics; Plant Proteins/metabolism; Polycyclic Sesquiterpenes; Polyisoprenyl Phosphates/metabolism; Sesquiterpenes/metabolism; Stereoisomerism; Substrate Specificity

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Proteins / Polyisoprenyl Phosphates / Sesquiterpenes / Diphosphates / Alkyl and Aryl Transferases / Mutation, Missense Language: En Journal: Biochemistry Year: 2016 Type: Article

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