Molecular impact of covalent modifications on nonribosomal peptide synthetase carrier protein communication.

Goodrich, Andrew C; Meyers, David J; Frueh, Dominique P

Goodrich, Andrew C; Meyers, David J; Frueh, Dominique P.

Affiliation

Goodrich AC; From the Department of Biophysics and Biophysical Chemistry and.
Meyers DJ; the Department of Pharmacology and Molecular Sciences Synthetic Core Facility, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Frueh DP; From the Department of Biophysics and Biophysical Chemistry and dfrueh1@jhmi.edu.

J Biol Chem ; 292(24): 10002-10013, 2017 06 16.

Article in En | MEDLINE | ID: mdl-28455448

Subject(s)

Bacterial Proteins/metabolism; Carrier Proteins/metabolism; Coenzyme A Ligases/metabolism; Models, Molecular; Peptide Synthases/metabolism; Protein Modification, Translational; Protein Processing, Post-Translational; Yersinia pestis/metabolism; Amino Acid Substitution; Apoenzymes/chemistry; Apoenzymes/genetics; Apoenzymes/metabolism; Apoproteins/chemistry; Apoproteins/genetics; Apoproteins/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Calorimetry; Carbon Isotopes; Carrier Proteins/chemistry; Carrier Proteins/genetics; Coenzyme A Ligases/chemistry; Coenzyme A Ligases/genetics; Fluorescence Polarization; Holoenzymes/chemistry; Holoenzymes/genetics; Holoenzymes/metabolism; Kinetics; Mutation; Nitrogen Isotopes; Nuclear Magnetic Resonance, Biomolecular; Peptide Synthases/chemistry; Peptide Synthases/genetics; Protein Conformation; Protein Interaction Domains and Motifs; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Titrimetry; Yersinia pestis/enzymology

Key words

adenylation domain; allostery; antibiotics; aryl carrier protein; nonribosomal peptide synthetase; nuclear magnetic resonance (NMR); protein dynamic; protein-protein interaction; structural biology

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptide Synthases / Bacterial Proteins / Yersinia pestis / Carrier Proteins / Models, Molecular / Protein Processing, Post-Translational / Coenzyme A Ligases / Protein Modification, Translational Language: En Journal: J Biol Chem Year: 2017 Type: Article

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