[Study on the Effect of Overexpression of miR-18a on Cellular Proliferation and Migration by Targeting ATM in Human Colorectal Cancer Cells].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 47(4): 451-457, 2016 Jul.
Article
in Zh
| MEDLINE
| ID: mdl-28591941
ABSTRACT
OBJECTIVES:
To study the regulation to colon cancer cellular biological properties through miR-18a targeting ataxia-telangiectasia mutated gene (ATM).METHODS:
A target of miR-18a was predicted by using bioinformatics tools. The miR-18a mimics and inhibitors were designed and synthesized. The expression of endogenous miR-18a in colon cancer cell line HCT116 was up-regulated or down-regulated by transfection. The effect of overexpression of miR-18a on cellular proliferation, invasion and migration via regulation of ATM gene expression was confirmed in vitro by using qRT-PCR, Western blot, MTT assay, clone forming assay and Transwell method, respectively.RESULTS:
ATM was identified as a potential target gene of miR-18a in the bioinformatics analysis. In addition, through transient transfection leading to the overexpression of miR-18a in HCT116 cell, the expression level of ATM was decreased. Down-regulation of HCT116 cell proliferation activity while significantly reducing HCT116 cell clone forming ability, lateral migration ability and longitudinal invasion ability were observed after transfected with miR-18a mimics. All of the changes were related to the overexpression of miR-18a.CONCLUSIONS:
miR-18a inhibited the proliferation and migration of colon cance cell HCT116 through negative regulation of ATM expression.Key words
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Colorectal Neoplasms
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Cell Movement
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MicroRNAs
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Cell Proliferation
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Ataxia Telangiectasia Mutated Proteins
Limits:
Humans
Language:
Zh
Journal:
Sichuan Da Xue Xue Bao Yi Xue Ban
Year:
2016
Type:
Article
Affiliation country:
China