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Optimizing T4 DNA polymerase conditions enhances the efficiency of one-step sequence- and ligation-independent cloning.
Islam, Mohammad Nazrul; Lee, Kyeong Won; Yim, Hyung-Soon; Lee, Seong Hyuk; Jung, Hae Chang; Lee, Jung-Hyun; Jeong, Jae-Yeon.
Affiliation
  • Islam MN; Marine Biotechnology Research Center, Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.
  • Lee KW; Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, Republic of Korea.
  • Yim HS; Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.
  • Lee SH; Marine Biotechnology Research Center, Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.
  • Jung HC; Marine Biotechnology Research Center, Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.
  • Lee JH; Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, Republic of Korea.
  • Jeong JY; Marine Biotechnology Research Center, Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.
Biotechniques ; 63(3): 125-130, 2017 09 01.
Article in En | MEDLINE | ID: mdl-28911316
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viral Proteins / Cloning, Molecular / Sequence Analysis, DNA / DNA-Directed DNA Polymerase Language: En Journal: Biotechniques Year: 2017 Type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viral Proteins / Cloning, Molecular / Sequence Analysis, DNA / DNA-Directed DNA Polymerase Language: En Journal: Biotechniques Year: 2017 Type: Article