Detection and complete genome characteristics of Posavirus 1 from pigs in China.
Virus Genes
; 54(1): 145-148, 2018 Feb.
Article
in En
| MEDLINE
| ID: mdl-29181630
ABSTRACT
Porcine stool-associated RNA virus 1 (Posavirus 1) is a novel member of picornaviruses and first identified from fecal samples of 30-day-old pigs with diarrhea in USA in 2011. To evaluate the existence of Posavirus 1 in swine herds, 118 clinical samples from diarrheal pigs and 31 fecal swabs from healthy pigs were collected and detected by reverse transcription-polymerase chain reaction (RT-PCR) using Posavirus 1-specific primers. Only five fecal samples from diarrheal pigs on two swine farms were positive for Posavirus 1. The complete genome sequences [excluding poly (A) tail] of two representative isolates SDQD-25 and HBTS-11 are determined and consist of 9840 and 9819 nucleotides in length, and encode one putative polyprotein of 3070 and 2952 amino acids, respectively. They share 90.3% homology with each other and 81.3-95.4% homologies with American Posavirus 1 isolates or strains at the nucleotide sequence level. The phylogenetic analysis based on the entire genomes of reference picornavirus strains or isolates showed SDQD-25, HBTS-11 cluster together with American Posavirus 1 isolates or strains, yet are clearly distant from the other picornaviruses. The complete genome sequences of Chinese Posavirus 1 isolates will enrich the information of Posavirus 1 sequence database and further expedite posavirus research on the genetic diversity, epidemiology, and evolution in China.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Picornaviridae
/
Swine Diseases
/
Picornaviridae Infections
/
Diarrhea
/
Feces
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
Virus Genes
Journal subject:
BIOLOGIA MOLECULAR
/
VIROLOGIA
Year:
2018
Type:
Article
Affiliation country:
China