Thermodynamic characterization of the multivalent interactions underlying rapid and selective translocation through the nuclear pore complex.

Hayama, Ryo; Sparks, Samuel; Hecht, Lee M; Dutta, Kaushik; Karp, Jerome M; Cabana, Christina M; Rout, Michael P; Cowburn, David

Hayama, Ryo; Sparks, Samuel; Hecht, Lee M; Dutta, Kaushik; Karp, Jerome M; Cabana, Christina M; Rout, Michael P; Cowburn, David.

Affiliation

Hayama R; From the Laboratory of Cellular and Structural Biology, Rockefeller University, New York, New York 10065.
Sparks S; the Departments of Biochemistry and of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, and.
Hecht LM; From the Laboratory of Cellular and Structural Biology, Rockefeller University, New York, New York 10065.
Dutta K; the New York Structural Biology Center, New York, New York 10027.
Karp JM; the Departments of Biochemistry and of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, and.
Cabana CM; From the Laboratory of Cellular and Structural Biology, Rockefeller University, New York, New York 10065.
Rout MP; From the Laboratory of Cellular and Structural Biology, Rockefeller University, New York, New York 10065, rout@mail.rockefeller.edu.
Cowburn D; the Departments of Biochemistry and of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, and cowburn@cowburnlab.org.

J Biol Chem ; 293(12): 4555-4563, 2018 03 23.

Article in En | MEDLINE | ID: mdl-29374059

Subject(s)

Cell Nucleus/metabolism; Intrinsically Disordered Proteins/metabolism; Nuclear Pore Complex Proteins/metabolism; Nuclear Pore/physiology; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae/metabolism; Thermodynamics; Active Transport, Cell Nucleus; Crystallography, X-Ray; Glycine/chemistry; Intrinsically Disordered Proteins/chemistry; Nuclear Pore Complex Proteins/chemistry; Phenylalanine/chemistry; Protein Binding; Protein Transport; Saccharomyces cerevisiae Proteins/chemistry

Key words

FG Nups/FG repeats; Phe-Gly nucleoporin; avidity; intrinsically disordered protein; isothermal titration calorimetry (ITC); multifunctional protein; multivalent interactions/fuzzy interactions; nuclear magnetic resonance (NMR); nuclear pore; nuclear pore complex; nuclear transport; nuclear transport/NTF2; protein complex; transport; transport factor

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Saccharomyces cerevisiae / Thermodynamics / Cell Nucleus / Nuclear Pore / Nuclear Pore Complex Proteins / Saccharomyces cerevisiae Proteins / Intrinsically Disordered Proteins Type of study: Prognostic_studies Language: En Journal: J Biol Chem Year: 2018 Type: Article

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