Exposure of helices &#945;4 and &#945;5 is required for insecticidal activity of Cry2Ab by promoting assembly of a prepore oligomeric structure.

Xu, Lian; Pan, Zhi-Zhen; Zhang, Jing; Niu, Li-Yang; Li, Jie; Chen, Zheng; Liu, Bo; Zhu, Yu-Jing; Chen, Qing-Xi

Exposure of helices α4 and α5 is required for insecticidal activity of Cry2Ab by promoting assembly of a prepore oligomeric structure.

Xu, Lian; Pan, Zhi-Zhen; Zhang, Jing; Niu, Li-Yang; Li, Jie; Chen, Zheng; Liu, Bo; Zhu, Yu-Jing; Chen, Qing-Xi.

Affiliation

Xu L; State Key Laboratory of Cellular Stress Biology, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, People's Republic of China.
Pan ZZ; Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, People's Republic of China.
Zhang J; State Key Laboratory of Cellular Stress Biology, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, People's Republic of China.
Niu LY; State Key Laboratory of Cellular Stress Biology, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, People's Republic of China.
Li J; State Key Laboratory of Cellular Stress Biology, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, People's Republic of China.
Chen Z; Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, People's Republic of China.
Liu B; Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, People's Republic of China.
Zhu YJ; Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, People's Republic of China.
Chen QX; State Key Laboratory of Cellular Stress Biology, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, People's Republic of China.

Cell Microbiol ; 20(6): e12827, 2018 06.

Article in En | MEDLINE | ID: mdl-29380507

Subject(s)

Bacterial Proteins/pharmacology; Endotoxins/pharmacology; Hemolysin Proteins/pharmacology; Insecticides/pharmacology; Lepidoptera/drug effects; Animals; Bacillus thuringiensis Toxins; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; DNA Mutational Analysis; Endotoxins/chemistry; Endotoxins/genetics; Endotoxins/metabolism; Hemolysin Proteins/chemistry; Hemolysin Proteins/genetics; Hemolysin Proteins/metabolism; Insecticides/chemistry; Insecticides/metabolism; Molecular Weight; Mutagenesis, Site-Directed; Mutant Proteins/chemistry; Mutant Proteins/genetics; Mutant Proteins/metabolism; Mutant Proteins/pharmacology; Protein Binding; Protein Conformation; Protein Multimerization; Proteolysis; Sequence Deletion

Key words

Cry2Ab; helices a4-a5; insecticidal activity; oligomerization; receptor binding; site-directed mutagenesis

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Endotoxins / Hemolysin Proteins / Insecticides / Lepidoptera Limits: Animals Language: En Journal: Cell Microbiol Journal subject: MICROBIOLOGIA Year: 2018 Type: Article

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