Streamlined Synthesis and Evaluation of Teichoic Acid Fragments.
Chemistry
; 24(16): 4014-4018, 2018 Mar 15.
Article
in En
| MEDLINE
| ID: mdl-29389054
ABSTRACT
Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages. Because of their microhetereogeneity, pure well-defined TAs for biological or immunological evaluation cannot be obtained from natural sources. We present here a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers. Building on the use of a "universal" linker system and fluorous tag purification strategy, a library of glycerolphosphate pentadecamers, decorated with various carbohydrate appendages, is generated. These are used to create a structurally diverse TA-microarray, which is used to reveal, for the first time, the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Teichoic Acids
Language:
En
Journal:
Chemistry
Journal subject:
QUIMICA
Year:
2018
Type:
Article
Affiliation country:
Netherlands