Ghrelin promotes human non-small cell lung cancer A549 cell proliferation through PI3K/Akt/mTOR/P70S6K and ERK signaling pathways.
Biochem Biophys Res Commun
; 498(3): 616-620, 2018 04 06.
Article
in En
| MEDLINE
| ID: mdl-29524402
ABSTRACT
Ghrelin is a gastric acyl-peptide that plays an important role in cell proliferation. In the present study, we explored the role of ghrelin in A549â¯cell proliferation and the possible molecular mechanisms. We found that ghrelin promotes A549â¯cell proliferation, knockdown of the growth hormone secretagogue receptor (GHSR) attenuated A549â¯cell proliferation caused by ghrelin. Ghrelin induced the rapid phosphorylation of phosphatidylinositol 3-kinase (PI3K), Akt, ERK, mammalian target of rapamycin (mTOR) and P70S6K. PI3K inhibitor (LY 294002), ERK inhibitor (PD98059) and mTOR inhibitor (Rapamycin) inhibited ghrelin-induced A549â¯cell proliferation. Moreover, GHSR siRNA inhibited phosphorylation of PI3K, Akt, ERK, mTOR and P70S6K induced by ghrelin. Akt and mTOR/P70S6K phosphorylation was inhibited by LY 294002 but not by PD98059. These results indicate that ghrelin promotes A549â¯cell proliferation via GHSR-dependent PI3K/Akt/mTOR/P70S6K and ERK signaling pathways.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Signal Transduction
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Carcinoma, Non-Small-Cell Lung
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Cell Proliferation
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Ghrelin
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Lung Neoplasms
Limits:
Humans
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2018
Type:
Article