Crystallization and preliminary X-ray studies of T4 phage beta-glucosyltransferase.

ABSTRACT

Crystals of the DNA glucosylating enzyme beta-glucosyltransferase from phage T4 have been grown in the presence of uridine diphosphate glucose. The crystals are orthorhombic, space group P2(1)2(1)2 with a = 148.3 A, b = 52.6 A, c = 52.6 A. The assumption of one monomer of Mr 40,000 per asymmetric unit gives rise to a Vm of 2.56 A 3/dalton. The crystals diffract to beyond 2.7 A and are suitable for X-ray structure analysis.