The Reaction Mechanism of Metallo-ß-Lactamases Is Tuned by the Conformation of an Active-Site Mobile Loop.

Palacios, Antonela R; Mojica, María F; Giannini, Estefanía; Taracila, Magdalena A; Bethel, Christopher R; Alzari, Pedro M; Otero, Lisandro H; Klinke, Sebastián; Llarrull, Leticia I; Bonomo, Robert A; Vila, Alejandro J

Palacios, Antonela R; Mojica, María F; Giannini, Estefanía; Taracila, Magdalena A; Bethel, Christopher R; Alzari, Pedro M; Otero, Lisandro H; Klinke, Sebastián; Llarrull, Leticia I; Bonomo, Robert A; Vila, Alejandro J.

Affiliation

Palacios AR; Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET-UNR), Ocampo y Esmeralda, Rosario, Argentina.
Mojica MF; Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
Giannini E; Research Service, Louis Stokes Veterans Affairs Medical Center, Cleveland, Ohio, USA.
Taracila MA; Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET-UNR), Ocampo y Esmeralda, Rosario, Argentina.
Bethel CR; Research Service, Louis Stokes Veterans Affairs Medical Center, Cleveland, Ohio, USA.
Alzari PM; Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
Otero LH; Research Service, Louis Stokes Veterans Affairs Medical Center, Cleveland, Ohio, USA.
Klinke S; Institut Pasteur, Unite de Microbiologie Structurale, CNRS UMR 3528/Université Paris Diderot, Paris, France.
Llarrull LI; Fundación Instituto Leloir, IIBBA-CONICET, Buenos Aires, Argentina.
Bonomo RA; Plataforma Argentina de Biología Estructural y Metabolómica PLABEM, Buenos Aires, Argentina.
Vila AJ; Fundación Instituto Leloir, IIBBA-CONICET, Buenos Aires, Argentina.

Antimicrob Agents Chemother ; 63(1)2019 01.

Article in En | MEDLINE | ID: mdl-30348667

Subject(s)

Anti-Bacterial Agents/chemistry; Ceftazidime/chemistry; Imipenem/chemistry; Meropenem/chemistry; Zinc/chemistry; beta-Lactamases/chemistry; Amino Acid Sequence; Anti-Bacterial Agents/metabolism; Catalytic Domain; Cefepime/chemistry; Cefepime/metabolism; Cefotaxime/chemistry; Cefotaxime/metabolism; Ceftazidime/metabolism; Cloning, Molecular; Crystallography, X-Ray; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression; Genetic Vectors/chemistry; Genetic Vectors/metabolism; Imipenem/metabolism; Kinetics; Meropenem/metabolism; Models, Molecular; Piperacillin/chemistry; Piperacillin/metabolism; Protein Binding; Protein Conformation, alpha-Helical; Protein Conformation, beta-Strand; Protein Engineering; Protein Interaction Domains and Motifs; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Substrate Specificity; Zinc/metabolism; beta-Lactam Resistance; beta-Lactamases/genetics; beta-Lactamases/metabolism

Key words

New Delhi metallo-ß-lactamase; antibiotic resistance; enzyme mechanism; enzyme structure; metallo-ß-lactamase

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Zinc / Beta-Lactamases / Ceftazidime / Imipenem / Meropenem / Anti-Bacterial Agents Language: En Journal: Antimicrob Agents Chemother Year: 2019 Type: Article Affiliation country: Argentina

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