The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA.

Iwasaki, Shintaro; Iwasaki, Wakana; Takahashi, Mari; Sakamoto, Ayako; Watanabe, Chiduru; Shichino, Yuichi; Floor, Stephen N; Fujiwara, Koichi; Mito, Mari; Dodo, Kosuke; Sodeoka, Mikiko; Imataka, Hiroaki; Honma, Teruki; Fukuzawa, Kaori; Ito, Takuhiro; Ingolia, Nicholas T

Iwasaki, Shintaro; Iwasaki, Wakana; Takahashi, Mari; Sakamoto, Ayako; Watanabe, Chiduru; Shichino, Yuichi; Floor, Stephen N; Fujiwara, Koichi; Mito, Mari; Dodo, Kosuke; Sodeoka, Mikiko; Imataka, Hiroaki; Honma, Teruki; Fukuzawa, Kaori; Ito, Takuhiro; Ingolia, Nicholas T.

Affiliation

Iwasaki S; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research, Wako, Saitama 351-0198, Japan; Department of Computational Biology and Medical Sciences, Graduate School of Frontier Scie
Iwasaki W; Laboratory for Translation Structural Biology, RIKEN Center for Biosystems Dynamics Research, Tsurumi-ku, Yokohama 230-0045, Japan; Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Tsurumi-ku, Yokohama 230-0045, Japan.
Takahashi M; Laboratory for Translation Structural Biology, RIKEN Center for Biosystems Dynamics Research, Tsurumi-ku, Yokohama 230-0045, Japan; Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Tsurumi-ku, Yokohama 230-0045, Japan.
Sakamoto A; Laboratory for Translation Structural Biology, RIKEN Center for Biosystems Dynamics Research, Tsurumi-ku, Yokohama 230-0045, Japan; Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Tsurumi-ku, Yokohama 230-0045, Japan.
Watanabe C; Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Tsurumi-ku, Yokohama 230-0045, Japan; Laboratory for Structure-Based Molecular Design, RIKEN Center for Biosystems Dynamics Research, Tsurumi-ku, Yokohama 230-0045, Japan.
Shichino Y; RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research, Wako, Saitama 351-0198, Japan.
Floor SN; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94143, USA; Helen Diller Family Comprehensive Cancer Center, University of California, San Francisc
Fujiwara K; Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research, Wako, Saitama 351-0198, Japan.
Mito M; RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research, Wako, Saitama 351-0198, Japan.
Dodo K; Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research, Wako, Saitama 351-0198, Japan; AMED-CREST, Japan Agency for Medical Research and Development, Wako, Saitama 351-0198, Japan; RIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, Japan.
Sodeoka M; Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research, Wako, Saitama 351-0198, Japan; AMED-CREST, Japan Agency for Medical Research and Development, Wako, Saitama 351-0198, Japan; RIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, Japan.
Imataka H; Graduate School of Engineering, University of Hyogo, Himeji, Hyogo 671-2201, Japan.
Honma T; Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Tsurumi-ku, Yokohama 230-0045, Japan; Laboratory for Structure-Based Molecular Design, RIKEN Center for Biosystems Dynamics Research, Tsurumi-ku, Yokohama 230-0045, Japan.
Fukuzawa K; School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Shinagawa, Tokyo 142-8501, Japan.
Ito T; Laboratory for Translation Structural Biology, RIKEN Center for Biosystems Dynamics Research, Tsurumi-ku, Yokohama 230-0045, Japan; Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Tsurumi-ku, Yokohama 230-0045, Japan. Electronic address: takuhiro.ito@riken.j
Ingolia NT; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: ingolia@berkeley.edu.

Mol Cell ; 73(4): 738-748.e9, 2019 02 21.

Article in En | MEDLINE | ID: mdl-30595437

Subject(s)

Benzofurans/metabolism; Eukaryotic Initiation Factor-4A/metabolism; Protein Biosynthesis; Protein Synthesis Inhibitors/metabolism; RNA/metabolism; Ribosomes/metabolism; Adenylyl Imidodiphosphate/chemistry; Adenylyl Imidodiphosphate/metabolism; Aglaia/chemistry; Aglaia/genetics; Aglaia/metabolism; Amino Acid Substitution; Benzofurans/chemistry; Benzofurans/isolation & purification; Benzofurans/pharmacology; Binding Sites; Drug Resistance/genetics; Eukaryotic Initiation Factor-4A/chemistry; Eukaryotic Initiation Factor-4A/genetics; HEK293 Cells; Humans; Models, Molecular; Molecular Structure; Mutation; Plant Proteins/chemistry; Plant Proteins/genetics; Plant Proteins/metabolism; Protein Binding; Protein Biosynthesis/drug effects; Protein Biosynthesis/genetics; Protein Interaction Domains and Motifs; Protein Synthesis Inhibitors/chemistry; Protein Synthesis Inhibitors/isolation & purification; Protein Synthesis Inhibitors/pharmacology; RNA/chemistry; Ribosomes/chemistry; Ribosomes/drug effects; Ribosomes/genetics; Structure-Activity Relationship

Key words

FMO; Ribo-Seq; Rocaglamide; X-ray; crystal structure; eIF4A; evolution; ribosome profiling; sequence specificity; translation

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ribosomes / Protein Biosynthesis / Benzofurans / RNA / Protein Synthesis Inhibitors / Eukaryotic Initiation Factor-4A Type of study: Prognostic_studies Language: En Journal: Mol Cell Journal subject: BIOLOGIA MOLECULAR Year: 2019 Type: Article

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